In the fourth film in this exciting series, and the first since losing his left eye, Yagyu Jubei is charged again with the task of protecting the Yagyu Secret Scrolls. The two scrolls are held in separate locations to assure their safety. Within them there is information other than the techniques of the Yagyu Shin-kage style. They contain a secret that could throw the country into turmoil. Clan founder Yagyu Sekishusai had split the scroll into two parts to prevent its contents from being known to the public. This is Jubei's toughest assignment yet as he puts his life on the line to save the nation!

This time, sword-saint Yagyu Jubei battles the evil corruption in Akizuki Village, with the help of his friend, a samurai named Matashichiro. This spaghetti western style action swordplay film is exciting and dynamic from start to finish! One-eyed master of martial arts, Yagyu Jubei visits Akizuki Village and meets with his former teacher Akizuki Hayato-no-Kami. Jubei is shocked to see Hayato's sickly appearance. An evil gang has plotted to murder Hayato, but a deadly Yagyu blade will unleash a rain of blood and disperse the evil plot! The breathtaking climax in a huge ravine called Okamidani is the ultimiate pay-off. Jubei confronts several hundred riflemen and warriors in a bloody battle to the death.

江户幕府时期，为牵制非亲藩地方外样大名，德川幕府决定从西班牙购入2500支新式火枪，但装货的御用船在海上被一伙来历不明的水军偷袭，这批枪被抢劫一空。幕府大怒，命柳生十兵卫尽早破案查明真相。十兵卫通过武艺帐忍之卷召集全国各地64名柳生门人齐聚江户，等待他们的，将是一场残酷的恶战。。 。

黑魔幫被次郎（電腦奇俠）毀滅後三年，已死的電腦黑魔竟率領黑魔部隊開始征服世界。他們為了實現野心而追捕少年阿明(アキラ)，因他手中掌握著某些秘密。
此時被封印在仁王像中的電腦奇俠01，感覺邪惡活動的回路開始啟動。最後他由仁王像中爆裂而出，為世界的正義而戰。