前編に続き、本懐を遂げた四十七士の自害までを描く。物語も佳境に入り、ワン・シーン＝ワン・ショットを徹底させた溝口演出はさらに重厚さを増す。戦線の拡大で物資が窮乏するなか、厖大な製作費を投入したこの作品は、破格の大作として完成を見た。前編に続き、新藤兼人がセット建築を担当している。

In 1701, Lord Takuminokami Asano has a feud with Lord Kira and he tries to kill Kira in the corridors of the Shogun's palace. The Shogun sentences Lord Asano to commit suppuku and deprives the palace and lands from his clan, but does not punish Lord Kira. Lord Asano's vassals leave the land and his samurais become ronin and want to seek revenge against the dishonor of their Lord. But their leader Kuranosuke Oishi asks the Shogun to restore the Asano clan with his brother Daigaku Asano. One year later, the Shogun refuses his request and Oishi and forty-six ronin revenge their Lord.